All lanes : Anti-MAP3K12 binding inhibitory protein 1 antibody [EPR13951] (ab186750) at 1/10000 dilutionLane 1 : Hela cell lysateLane 2 : 293 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
All lanes : Anti-MAP3K12 binding inhibitory protein 1 antibody [EPR13951] (ab186750) at 1/10000 dilutionLane 1 : Human fetal lung lysateLane 2 : Human fetal heart lysateLysates/proteins at 10 µg per lane.SecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling MAP3K12 binding inhibitory protein 1 with ab186750 at 1/250 dilution. Secondary antibody: Ready to use HRP Polymer for Rabbit IgG. Counter stain: Hematoxylin.
Immunohistochemical analysis of paraffin-embedded Human clear cell carcinoma of kidney tissue labeling MAP3K12 binding inhibitory protein 1 with ab186750 at 1/250 dilution. Secondary ab: ready to use HRP Polymer for Rabbit IgG. Counter stain: Hematoxylin.
Immunofluorescence analysis of HeLa cells labeling MAP3K12 binding inhibitory protein 1 with ab186750 at 1/500 dilution. Secondary antibody: Goat anti rabbit IgG (Alexa Fluor® 488). Fixative: 4% paraformaldehyde. Counter stain: Dapi (blue).
Flow cytometric analysis of HeLa cell labeling MAP3K12 binding inhibitory protein 1 using ab186750 at 1/400 dilution. Secondary antibody: Goat anti rabbit IgG (FITC) at 1/400 dilution. Fixative: 2% paraformaldehyde. Isotype control: Rabbit monoclonal IgG.