All lanes : Anti-LRRC20 antibody [EP9977(2)] (ab186416) at 1/2000 dilutionLane 1 : PC3 cell lysateLane 2 : Jurkat cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
All lanes : Anti-LRRC20 antibody [EP9977(2)] (ab186416) at 1/2000 dilutionLane 1 : Rat heart tissue lysateLane 2 : C6 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Western blot analysis of LRRC20 in immunoprecipitation pellets from PC3 lysate. Lane 1: ab186416 used at 1/50 dilution, Lane 2: PBS negative control. Secondary antibody Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG