All lanes : Anti-LFA3 antibody [EP15041] (ab196648) at 1/5000 dilutionLane 1 : Raji (Human Burkitt's lymphoma cell line) whole cell lysateLane 2 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
All lanes : Anti-LFA3 antibody [EP15041] (ab196648) at 1/1000 dilutionLane 1 : TF-1 (Human bone marrow erythroleukemia cells) whole cell lysateLane 2 : K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
All lanes : Anti-LFA3 antibody [EP15041] (ab196648) at 1/2000 dilutionLane 1 : SW480 (Human colorectal adenocarcinoma cell line) controlLane 2 : SW480 (Human colorectal adenocarcinoma cell line) de-glycosylationLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution