Lane 1 : Anti-Lamin A + C (mutated R482 W) antibody [5H8-B4] (ab176549) at 1/50 dilutionLane 2 : Anti-Lamin A + C (mutated R482 W) antibody [5H8-B4] (ab176549) at 1/200 dilutionLane 3 : Anti-Lamin A + C (mutated R482 W) antibody [5H8-B4] (ab176549) at 1/1000 dilutionLane 4 : Anti-Lamin A + C (mutated R482 W) antibody [5H8-B4] (ab176549) at 1/2000 dilutionLane 5 : Anti-Lamin A + C (mutated R482 W) antibody [5H8-B4] (ab176549) at 1/4000 dilutionLane 6 : Anti-Lamin A + C (mutated R482 W) antibody [5H8-B4] (ab176549) at 1/8000 dilutionLane 7 : Anti-Lamin A + C (mutated R482 W) antibody [5H8-B4] (ab176549) at 1/16000 dilutionLane 8 : Anti-Lamin A + C (mutated R482 W) antibody [5H8-B4] (ab176549) at 1/32000 dilutionLane 1 : HeLa cell lysateLane 2 : HeLa cell lysateLane 3 : HeLa cell lysateLane 4 : HeLa cell lysateLane 5 : HeLa cell lysateLane 6 : HeLa cell lysateLane 7 : HeLa cell lysateLane 8 : HeLa cell lysateSecondaryAnti-mouse IgG (whole molecule)-AP conjugate at 1/2000 dilution