
Western blot analysis of c-Jun p39 expression in untreated (A,C) and phorbol ester-induced (B,D) NIH/3T3 nuclear extracts. Antibodies tested include c-Jun (D): sc-44 (A,B) and c-Jun (N): sc-45 (C,D).

c-Jun (D): sc-44. Western blot analysis of c-Jun expression in Jurkat nuclear extract.

ChiP analysis of transcription factor binding to the CD28 responsive positive regulatory region IV (PRRIV) in resting (A,B) and CD3+CD28-stimulated human primary T cells. 10% (A,C) and 1% (B,D) of input DNAs were amplified. Antibodies used included c-Jun (D): sc-44; c-Fos (6-2H-2F): sc-447 and Ets-1/Ets-2 (C-275): sc-112. Data kindly provided by J. Imbert and reproduced with permission from Yeh, J.H., et al, Mol. Cell. Biol., 2001, 21:4515-4527.

c-Jun siRNA (m): sc-29224. Western blot analysis of c-Jun expression in nontransfectedcontrol (A) and c-Jun siRNA transfected (B) NIH/3T3 cells. Blot probed with c-Jun: sc-44. GAPDH (FL-335): sc-25778 used as specificity and loading control.

c-Jun (D): sc-44. Western blot analysis of c-Jun expression in non-transfected (A) and human c-Jun transfected (B) CHO whole cell lysates.

c-Jun (D): sc-44. Western blot analysis of c-Jun expression in non-transfected: sc-110760 (A) and human c-Jun transfected: sc-110759 (B) 293 whole cell lysates.

c-Jun (D): sc-44. Western blot analysis of c-Jun expression in non-transfected: sc-117752 (A) and mouse c-Jun transfected: sc-125069 (B) 293T whole cell lysates.

c-Jun (D): sc-44. Western blot analysis of c-Jun expression in non-transfected: sc-117752 (A) and mouse c-Jun transfected: sc-125070 (B) 293T whole cell lysates.

c-Jun (D): sc-44. Western blot analysis of c-Jun expression in non-transfected 293T: sc-117752 (A), mouse c-Jun transfected 293T: sc-125071 (B) and NIH/3T3 (C) whole cell lysates.

c-Jun (D): sc-44. Western blot analysis of Jun D expression in non-transfected: sc-117752 (A) and mouse Jun D transfected: sc-121171 (B) 293T whole cell lysates and NIH/3T3 nuclear extract (C).

c-Jun (D): sc-44. Western blot analysis of c-Jun expression in non-transfected CHO: sc-117750 (A), human c-Jun transfected CHO: sc-110019 (B) and NIH/3T3 (C) whole cell lysates.

c-Jun (D): sc-44. Western blot analysis of c-Jun expression in non-transfected 293T: sc-117752 (A), human c-Jun transfected 293T: sc-159456 (B) and UV irradiated HeLa (C) whole cell lysates.