Anti-Lactate Dehydrogenase Isoenzyme V antibody

Name	Anti-Lactate Dehydrogenase Isoenzyme V antibody
Supplier	Abcam
Catalog	ab85472
Prices	$376.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Applications	ICC/IF ICC/IF WB
Species Reactivities	Mouse, Rat, Human, Chimpanzee, Orangutan
Antigen	Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Lactate Dehydrogenase Isoenzyme V
Description	Rabbit Polyclonal
Gene	LDHA
Conjugate	Unconjugated
Supplier Page	Shop

Product images

All lanes : Anti-Lactate Dehydrogenase Isoenzyme V antibody (ab85472) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 : Kidney (Human) Tissue Lysate - adult normal tissue (ab30203)Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate Lane 4 : Liver (Human) Tissue Lysate - adult normal tissue (ab29889)Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate Lane 6 : Cervix (Human) Whole Cell Lysate - adult normal tissue (ab29226)Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

ICC/IF image of ab85472 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab85472, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Lactate Dehydrogenase Isoenzyme V antibody (ab85472) at 1 µg/ml + Active human Lactate Dehydrogenase Isoenzyme V full length protein (ab77945) at 0.1 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.Exposure time : 12 minutes

All lanes : Anti-Lactate Dehydrogenase Isoenzyme V antibody (ab85472) at 1 µg/mlLane 1 : Kidney (Mouse) Tissue LysateLane 2 : Liver (Mouse) Tissue Lysate Lane 3 : Liver (Rat) Tissue LysateLane 4 : Heart (Rat) Tissue Lysate Lysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.