All lanes : Anti-KSR1 (phospho S392) antibody [EPR2359(2)] (ab129111) at 1/1000 dilution (purified)Lane 1 : Untreated HEK293 cellsLane 2 : HEK293 cells treated with Calcyculin ALysates/proteins at 10 µg per lane.SecondaryHRP goat anti-rabbit (H+L) at 1/1000 dilution
All lanes : Anti-KSR1 (phospho S392) antibody [EPR2359(2)] (ab129111) at 1/1000 dilution (unpurified)Lane 1 : 293T cell lysate, untreatedLane 2 : 293T cell lysate treated with Calyculin ALysates/proteins at 10 µg per lane.SecondaryStandard HRP labelled goat anti-rabbit at 1/2000 dilution
Equilibrium disassociation constant (KD)Learn more about KD Click here to learn more about KD