Anti-KAT4 / TBP Associated Factor 1 antibody - ChIP Grade
| Name | Anti-KAT4 / TBP Associated Factor 1 antibody - ChIP Grade |
|---|---|
| Supplier | Abcam |
| Catalog | ab28450 |
| Prices | $385.00 |
| Sizes | 50 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P ELISA WB ChIP |
| Species Reactivities | Mouse, Human, Rat, Dog |
| Antigen | Synthetic peptide derived from residues 1823 - 1872 (C-terminal) of human TAF1 |
| Description | Rabbit Polyclonal |
| Gene | TAF1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Quiescent human colon carcinoma HCT116 cultures were treated with 10% FBS for three time points (0, 15, 30 minutes) or (0, 30, 60 minutes) were used in Matrix-ChIP and real-time PCR assays at EGR1 gene (Exon1) and 15kb upstream site.
This image shows human kidney stained with ab28450 at 8µg/ml. Magnification 400x.
Anti-KAT4 / TBP Associated Factor 1 antibody - ChIP Grade (ab28450) at 0.5 µg/ml + Daudi cell lysate
Chromatin was prepared from Hela cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10mins. The ChIP was performed with 25µg of chromatin, 8µg of ab28450 (blue), and 20µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman and sybr green approach). Primers and probes for the actively transcribed genes GAPDH and ACT1 are located in the core promoter.
Product References
Transforming growth factor-beta regulates basal transcriptional regulatory - Transforming growth factor-beta regulates basal transcriptional regulatory
Iwata J, Hosokawa R, Sanchez-Lara PA, Urata M, Slavkin H, Chai Y. J Biol Chem. 2010 Feb 12;285(7):4975-82.
Two modes of transcriptional activation at native promoters by NF-kappaB p65. - Two modes of transcriptional activation at native promoters by NF-kappaB p65.
van Essen D, Engist B, Natoli G, Saccani S. PLoS Biol. 2009 Mar 31;7(3):e73.