Sonicated chromatin prepared from U2OS cells was subjected to the ChIP procedure with ab2832 to CBP. Immunoprecipitated chromatin was analysed in the promoter region of c-FOS (active) and in exon 2 of MYO-D (inactive). Values are % of inputs.  2–4 µg of ab2832 and 1-2x106 cells were used in each ChIP experiment. Sergei Denissov, Prof. Henk Stunnenberg lab, University Nijmengen, NL

IHC image of KAT3A/CBP staining in human pancreas formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab2832, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.