All lanes : Anti-Kallikrein 6 antibody - Catalytic domain (ab40952) at 1/1000 dilutionLane 1 : Cell line A2058. SFLane 2 : Cell line A2058. TPALane 3 : Cell line A2058. IL1-alphaLane 4 : Cell line A2058. TNF-alphaLane 5 : Cell line Hs 294T. SFLane 6 : Cell line Hs 294T. TPALane 7 : Cell line Hs 294T. IL1-betaLane 8 : Cell line Hs 294T. TNF-alphaLane 9 : Cell line DU145. SFLane 10 : Cell line DU145. TPALane 11 : Cell line DU145. IL1-betaLane 12 : Cell line LMCaP. SFLane 13 : Cell line LMCaP. 10uM TestosteroneLysates/proteins at 0.01 µg per lane.