Anti-Kallikrein 13 antibody - Kallikrein loop
| Name | Anti-Kallikrein 13 antibody - Kallikrein loop |
|---|---|
| Supplier | Abcam |
| Catalog | ab28569 |
| Prices | $370.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB |
| Species Reactivities | Human |
| Antigen | Synthetic peptide based on the Kallikrein loop area of human Kallikrein 13 |
| Description | Rabbit Polyclonal |
| Gene | KLK13 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-Kallikrein 13 antibody - Kallikrein loop (ab28569) at 1/1000 dilutionLane 1 : Kallikrein 13 at 0.05 µgLane 2 : Kallikrein 13 at 0.01 µgLane 3 : Kallikrein 13 at 0.001 µg
ICC/IF image of ab28569 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28569, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.