Anti-Isocitrate dehydrogenase antibody [GT1521]

Name	Anti-Isocitrate dehydrogenase antibody [GT1521]
Supplier	Abcam
Catalog	ab184615
Prices	$384.00
Sizes	100 µl
Host	Mouse
Clonality	Monoclonal
Isotype	IgG2b
Clone	GT1521
Applications	ICC/IF ICC/IF IP WB
Species Reactivities	Mouse, Rat, Human, Monkey
Antigen	Recombinant fragment within Human Isocitrate dehydrogenase aa 30-337
Description	Mouse Monoclonal
Gene	IDH1
Conjugate	Unconjugated
Supplier Page	Shop

Product images

Immunoprecipitaion analysis:Lane1: 30 μg whole cell lysate/extract of Isocitrate dehydrogenase expressing HepG2 cells;Lane 2: Control with 2.5 μg of pre-immune mouse IgG;Lane 3: Immunoprecipitation of Isocitrate dehydrogenase protein by 2.5 μg of ab184615. The immunoprecipitated Isocitrate dehydrogenase protein was detected by western blot using ab184615 diluted at 1 : 1000.

Immunofluorescent analysis of ice-cold MeOH fixed (for 5 min) A431 cells labeling Isocitrate dehydrogenase at a 1/200 dilution (green), with Hoechst 33342 counterstaining (blue).

All lanes : Anti-Isocitrate dehydrogenase antibody [GT1521] (ab184615) at 1/1000 dilutionLane 1 : Neuro2A whole cell lysate/extractLane 2 : GL261 whole cell lysate/extractLane 3 : C8D30 whole cell lysate/extractLane 4 : NIH-3T3 whole cell lysate/extractLane 5 : Raw264.7 whole cell lysate/extractLane 6 : C2C12 whole cell lysate/extractLysates/proteins at 30 µg per lane.

All lanes : Anti-Isocitrate dehydrogenase antibody [GT1521] (ab184615) at 1/1000 dilutionLane 1 : PC-12 whole cell lysate/extractLane 2 : Rat-2 whole cell lysate/extractLysates/proteins at 30 µg per lane.

All lanes : Anti-Isocitrate dehydrogenase antibody [GT1521] (ab184615) at 1/1000 dilutionLane 1 : 293T whole cell lysate/extractLane 2 : A431 whole cell lysate/extractLane 3 : HeLa whole cell lysate/extractLane 4 : HepG2 whole cell lysate/extractLysates/proteins at 30 µg per lane.