Anti-Interferon gamma antibody
| Name | Anti-Interferon gamma antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab25101 |
| Prices | $385.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ELISA Secretion assay B/N ICC/IF WB IHC-F ELISA IHC-P |
| Species Reactivities | Human, Monkey |
| Antigen | Recombinant human IFN gamma derived from E |
| Description | Rabbit Polyclonal |
| Gene | IFNG |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Standard curve for Interferon gamma (Analyte: ab51240); dilution range 1pg/ml to 1µg/ml using Capture Antibody ab25014 at 5µg/ml and Detector Antibody ab25101 at 0.5µg/ml.
Ab25101 staining Human tonsil. Staining is localised to the cytoplasm and protein is secreted.Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
Anti-Interferon gamma antibody (ab25101) at 1 µg/ml + Human Interferon gamma full length protein (ab51240) at 0.01 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.Exposure time : 30 seconds
Product References
NK cell responses to simian immunodeficiency virus vaginal exposure in naive and - NK cell responses to simian immunodeficiency virus vaginal exposure in naive and
Shang L, Smith AJ, Duan L, Perkey KE, Qu L, Wietgrefe S, Zupancic M, Southern PJ, Masek-Hammerman K, Reeves RK, Johnson RP, Haase AT. J Immunol. 2014 Jul 1;193(1):277-84.
Involvement of different CD4(+) T cell subsets producing granzyme B in the immune - Involvement of different CD4(+) T cell subsets producing granzyme B in the immune
Naouar I, Boussoffara T, Ben Ahmed M, Belhaj Hmida N, Gharbi A, Gritli S, Ben Salah A, Louzir H. Mediators Inflamm. 2014;2014:636039.