Anti-Interferon beta antibody (Biotin)
| Name | Anti-Interferon beta antibody (Biotin) |
|---|---|
| Supplier | Abcam |
| Catalog | ab84258 |
| Prices | $385.00 |
| Sizes | 50 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ELISA WB IHC-P ICC/IF ICC/IF |
| Species Reactivities | Human |
| Antigen | Highly pure (>98%) recombinant human Interferon beta |
| Description | Rabbit Polyclonal |
| Gene | IFNB1 |
| Conjugate | Biotin |
| Supplier Page | Shop |
Product images
ICC/IF image of ab84258 stained HepG2 cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab84258, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
All lanes : Anti-Interferon beta antibody (Biotin) (ab84258) at 1 µg/mlLane 1 : Liver (Human) Tissue Lysate - adult normal tissue (ab29889)Lane 2 : Spleen (Human) Tissue Lysate - adult normal tissue (ab29699)Lane 3 : Kidney (Human) Tissue Lysate - adult normal tissue (ab30203)Lysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human normal placenta, staining Interferon beta with ab84258 at 0.75 µg/ml. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended.