Anti-Integrin alpha 5 antibody [EPR7854]

Name	Anti-Integrin alpha 5 antibody [EPR7854]
Supplier	Abcam
Catalog	ab150361
Prices	$397.00
Sizes	100 µl
Host	Rabbit
Clonality	Monoclonal
Isotype	IgG
Clone	EPR7854
Applications	WB IHC-P ICC/IF ICC/IF IP FC
Species Reactivities	Mouse, Rat, Human
Antigen	Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) (C terminal)
Description	Rabbit Monoclonal
Gene	ITGA5
Conjugate	Unconjugated
Supplier Page	Shop

Product images

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U937 (Human histiocytic lymphoma) cells labeling Integrin alpha 5 with ab150361 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor 488) (ab150077) secondary antibody at 1/400 dilution (green). Confocal image showing mainly membrane staining on U937 cell line. The nuclear counterstain is DAPI (blue).

All lanes : Anti-Integrin alpha 5 antibody [EPR7854] (ab150361) at 1/1000 dilutionLane 1 : Human bladder cell lysateLane 2 : HT-1080 cell lysateLane 3 : HeLa cell lysateLane 4 : U937 cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Immunohistochemical analysis of paraffin embedded Human kidney tissue labelling Integrin alpha 5 with ab150361 antibody at a dilution of 1/100.

Immunohistochemical analysis of paraffin embedded Human Vessels tissue using ab150361 showing +ve staining.

Immunohistochemical analysis of paraffin embedded Human Brain vessels tissue using ab150361 showing +ve staining.

Immunohistochemical analysis of paraffin embedded Human Skeletal muscle vessel tissue using ab150361 showing +ve staining.

Immunohistochemical analysis of paraffin embedded Normal Human Tonsil tissue using ab150361 showing +ve staining.

Immunohistochemical analysis of paraffin embedded Normal Human Uterus tissue using ab150361 showing +ve staining.

Overlay histogram showing HeLa cells stained with ab150361 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab150361, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was goat anti-rabbit Alexa Fluor 488 IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.