Anti-Integrin alpha 2+beta 1 antibody [16B4]
| Name | Anti-Integrin alpha 2+beta 1 antibody [16B4] |
|---|---|
| Supplier | Abcam |
| Catalog | ab30483 |
| Prices | $391.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | 16B4 |
| Applications | ICC/IF ICC/IF IP FC ELISA IHC-F |
| Species Reactivities | Human |
| Antigen | Purified human beta 1 preparation from HT1080 fibrosarcoma cell extract |
| Description | Mouse Monoclonal |
| Gene | ITGA2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Overlay histogram showing HT1080 cells stained with ab30483 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab30483, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was a goat anti-mouse Alexa Fluor® 488 (IgG; H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HT1080 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/8968_ab30483-4-ab30483FC.jpg)
Overlay histogram showing HT1080 cells stained with ab30483 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab30483, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was a goat anti-mouse Alexa Fluor® 488 (IgG; H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HT1080 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
ICC/IF image of ab30483 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab30483, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) MCF7 cells at 1µg/ml.
ab30483 staining human breast cancer cells by ICC/IF. Cells were PFA fixed and blocked with 1% serum for 16 hours at 20°C prior to incubating with ab30483 (at 30µg/ml) for 16 hours at 4°C. An Alexa Fluor® 488 conjugated goat anti-mouse antibody, diluted 1/200, was used as the secondary.See Abreview
Product References
Clonogenicity: holoclones and meroclones contain stem cells. - Clonogenicity: holoclones and meroclones contain stem cells.
Beaver CM, Ahmed A, Masters JR. PLoS One. 2014 Feb 26;9(2):e89834.
Syndecan-1 regulates vascular smooth muscle cell phenotype. - Syndecan-1 regulates vascular smooth muscle cell phenotype.
Chaterji S, Lam CH, Ho DS, Proske DC, Baker AB. PLoS One. 2014 Feb 25;9(2):e89824.
In vitro and in vivo characterization of the actin polymerizing compound - In vitro and in vivo characterization of the actin polymerizing compound
Menhofer MH, Bartel D, Liebl J, Kubisch R, Busse J, Wagner E, Muller R, Vollmar AM, Zahler S. Cardiovasc Res. 2014 Nov 1;104(2):303-14.
Monoclonal antibodies identify residues 199-216 of the integrin alpha2 vWFA - Monoclonal antibodies identify residues 199-216 of the integrin alpha2 vWFA
Tuckwell DS, Smith L, Korda M, Askari JA, Santoso S, Barnes MJ, Farndale RW, Humphries MJ. Biochem J. 2000 Sep 1;350 Pt 2:485-93.
Transmembrane 4 superfamily protein CD151 (PETA-3) associates with beta 1 and - Transmembrane 4 superfamily protein CD151 (PETA-3) associates with beta 1 and
Fitter S, Sincock PM, Jolliffe CN, Ashman LK. Biochem J. 1999 Feb 15;338 ( Pt 1):61-70.
PETA-3/CD151, a member of the transmembrane 4 superfamily, is localised to the - PETA-3/CD151, a member of the transmembrane 4 superfamily, is localised to the
Sincock PM, Fitter S, Parton RG, Berndt MC, Gamble JR, Ashman LK. J Cell Sci. 1999 Mar;112 ( Pt 6):833-44.