Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling INPPL1 with ab70267 at 1/1000 (1µg/ml). Detection: DAB.
Anti-INPPL1 antibody (ab70267) at 0.1 µg/ml + HeLa whole cell lysate at 50 µgdeveloped using the ECL technique
Immunocytochemistry/Immunofluorescence analysis of human GIST882 cells labelling INPPL1 (top) with ab70267. Bottom - Hoechst 33342.
Immunoprecipitation of HeLa whole cell lysate (1mg) using ab70267 at 3 µg/mg. 1/4 of the immunoprecipitate was loaded on to a gel and analysed by Western blot using ab70267 at 0.1 µg/ml. Blot was developed using chemiluminescence with an exposure time of 30 seconds.
IHC image of ab70267 staining in Human breast cancer formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab70267, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.