
All lanes : Anti-IL3RA antibody (ab128132) at 1 µg/mlLane 1 : Spleen (Human) Tissue Lysate - adult normal tissue (ab29699)Lane 2 : Thymus (Human) Tissue Lysate - adult normal tissue (ab30146)Lane 3 : Bone Marrow (Human) Tissue Lysate - adult normal tissueLane 4 : U937 (Human leukemic monocyte lymphoma cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.