
All lanes : Anti-IL36 alpha antibody (ab116279) at 1 µg/mlLane 1 : Brain (Human) Tissue Lysate - adult normal tissueLane 2 : Brain (Human) Tissue Lysate - fetal normal tissueLane 3 : Brain (Human) Tissue Lysate - adult normal tissue with Immunising peptide at 1 µg/mlLane 4 : Brain (Human) Tissue Lysate - fetal normal tissue with Immunising peptide at 1 µg/mlLysates/proteins at 25 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.