Btk (D3H5) Rabbit mAb

Name	Btk (D3H5) Rabbit mAb
Supplier	Cell Signaling Technology
Catalog	8547
Prices	$250.00
Sizes	100 µl (10 western blots)
Host	Rabbit
Clonality	Monoclonal
Isotype	IgG
Clone	D3H5
Applications	WB IP IHC-P FC
Species Reactivities	Human, Mouse, Rat, Hamster, Bovine, Dog, Pig, Horse
Antigen	Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp195 of human Btk protein.
Description	Rabbit Monoclonal
Gene	BTK
Supplier Page	Shop

Product images

Western blot analysis of extracts from various cell lines using Btk (D3H5) Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Btk (D3H5) Rabbit mAb. Note staining of inflammatory cells.

Immunohistochemical analysis of paraffin-embedded human B-cell lymphoma using Btk (D3H5) Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded mouse colon using Btk (D3H5) Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma using Btk (D3H5) Rabbit mAb. Note staining of inflammatory cells.

Immunohistochemical analysis of paraffin-embedded cell pellets, Ramos(left) or Jurkat (right), using Btk (D3H5) Rabbit mAb.

Human whole blood was fixed, lysed, and permeabilized as per the Cell Signaling Technology Flow Alternate Protocol and stained using Btk (D3H5) Rabbit mAb. Samples were co-stained using CD3-PE and CD19-APC to distinguish T and B cell subpopulations, respectively. B (red) and T (blue) cell population gates were applied to a histogram depicting the mean fluorescence intensity of Btk. Anti-rabbit IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 488 Conjugate) #4412 was used as a secondary antibody.