Lane 1 : Anti-IL17 antibody (ab79056) at 0.5 µg/mlLane 2 : Anti-IL17 antibody (ab79056) at 1 µg/mlLane 1 : A-20 cell lysateLane 2 : A-20 cell lysateLysates/proteins at 15 µg per lane.

ab79056 staining IL17 in Dog lymph node tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde, permeabilized with Tween-20 (0.05%) and blocked with 10% serum for 2 hours at 24°C; antigen retrieval was by heat mediation with citrate buffer (0.01M). Samples were incubated with primary antibody (1/100 in PBS +10% serum) for 14 hours at 4°C. A Biotin-conjugated Horse pan-specific anti-IgG polyclonal (1/20) was used as the secondary antibody.See Abreview