ab106732 staining IL13 in human intestinal tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).Tissue was fixed in formaldehyde, a heat mediated antigen retrieval step was performed using citrate buffer pH 6, samples were then incubated with ab106732 at a 1/800 dilution for 20 minutes at 25°C. The secondary used was an undiluted HRP polymer conjugated goat anti-rabbit/mouse polyclonal.See Abreview

ab106732 staining IL13 in murine colon tissue by Immunohistochemistry (Frozen sections). Tissue was fixed with paraformaldehyde and blocked with 5% serum for 20 hours at 25°C. Samples were incubated with primary antibody (1/100 in 1% BSA in PBS) for 16 hours at 4°C. An AlexaFluor®488-conjugated goat anti-rabbit polyclonal IgG (1/200) was used as the secondary antibody. See Abreview

Anti-IL13 antibody (ab106732) at 1/1000 dilution + Mouse liver tissue lysate at 100 µgSecondaryHRP-conjugated goat anti-rabbit polyclonal IgG at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.