Anti-IL10 antibody
| Name | Anti-IL10 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab9969 |
| Prices | $398.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB ELISA B/N ICC/IF ICC/IF |
| Species Reactivities | Mouse, Rat |
| Antigen | Recombinant fragment, corresponding to amino acids 19/178 of Rat IL10 (available as ab9970 |
| Description | Rabbit Polyclonal |
| Gene | Il10 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab9969 staining IL10 in RAW 246.7 cells treated with spermidine hydrochloride (ab120057), by ICC/IF. Increase in IL10 expression correlates with increased concentration of spermidine hydrochloride, as described in literature.The cells were incubated at 37°C for 24 hour in media containing different concentrations of ab120057 (spermidine hydrochloride) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab9969 (1 μg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
ab9969 staining IL10 in murine immortalised bone marrow-derived macrophage by Immunocytochemistry/ Immunofluorescence. The cells were fixed in paraformaldehyde, permeabilised in 0.01% Triton X-100 and then blocked using 5% serum for 1 hour at 20°C. Samples were then incubated with primary antibody at 1/200 for 1 hour at 20°C. The secondary antibody used was a goat anti-rabbit IgG conjugated to Alexa Fluor® 568 (red) used at a 1/500 dilution.See Abreview
ab9969 staining nucleolin in Raw 264.7 cells treated with spermine (ab120051), by ICC/IF. Increase in IL10 expression correlates with increased concentration of spermine, as described in literature.The cells were incubated at 37°C for 6h in media containing different concentrations of ab120051) (spermine) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab9969 (1 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
Product References
Chronic antidepressant desipramine treatment increases open field-induced brain - Chronic antidepressant desipramine treatment increases open field-induced brain
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Effects of acupuncture on Th1, th2 cytokines in rats of implantation failure. - Effects of acupuncture on Th1, th2 cytokines in rats of implantation failure.
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Acetate reduces microglia inflammatory signaling in vitro. - Acetate reduces microglia inflammatory signaling in vitro.
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Impaired induction of IL-10 expression in the lung following hemorrhagic shock. - Impaired induction of IL-10 expression in the lung following hemorrhagic shock.
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