Anti-IkB beta (phospho S23) antibody
| Name | Anti-IkB beta (phospho S23) antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab51142 |
| Prices | $370.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB ELISA |
| Species Reactivities | Human |
| Antigen | Synthetic phosphopeptide derived from human IkB-ß around the phosphorylation site of serine 23 (L-G-SP-L-G) |
| Description | Rabbit Polyclonal |
| Gene | NFKBIB |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-IkB beta (phospho S23) antibody (ab51142) at 1/300 dilutionLane 1 : extracts from Hela cells treated with TNF-a with immunizing peptideLane 2 : extracts from Hela cells treated with TNF-aLysates/proteins at 0.02 µg/ml per lane.
ICC/IF image of ab51142 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51142, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.