
Anti-IGLL1 antibody (ab67081) at 1/500 dilution + Human kidney tissue lysate at 25 µg/mlSecondaryGoat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilutionObserved band size : 29 kDa (why is the actual band size different from the predicted?)Additional bands at : 58 kDa. We are unsure as to the identity of these extra bands.

All lanes : Anti-IGLL1 antibody (ab67081) at 1/500 dilutionLane 1 : IGLL1 transfected 293T cell lysateLane 2 : Non-transfected 293T cell lysateLysates/proteins at 25 µg/ml per lane.SecondaryGoat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilutionObserved band size : 24 kDa (why is the actual band size different from the predicted?)Additional bands at : 16 kDa. We are unsure as to the identity of these extra bands.