Immunofluorescent staining of Hydroxysteroid (17-beta) Dehydrogenase 4 in HDFn cells (human) using ab126565. The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15min). The cells were then incubated with the antibody (ab128565, 1µg/ml) for 2h at room temperature or over night at 4°C. The secondary antibody was (red) Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. 10% Goat serum was used as the blocking agent for all blocking steps. The target protein locates to the peroxisome.

All lanes : Anti-Hydroxysteroid (17-beta) Dehydrogenase 4 antibody [2D3BB5BF10] (ab128565) at 1 µg/mlLane 1 : ladderLane 2 : Human Liver Homoginate at 10 µgLane 3 : Human Heart Homogenate at 10 µgLane 4 : Hela cell lysate at 10 µgLane 5 : HepG2 cell lysate at 10 µgLane 6 : HDFn cell lysate at 10 µgLane 7 : Hek293T cell lysate at 10 µgLane 8 : Jurket cell lysate at 10 µgLane 9 : SH-SY5Y cell lysate at 10 µg