Anti-HSPA4 antibody [EPR14166]

Name	Anti-HSPA4 antibody [EPR14166]
Supplier	Abcam
Catalog	ab185962
Prices	$357.00
Sizes	100 µl
Host	Rabbit
Clonality	Monoclonal
Isotype	IgG
Clone	EPR14166
Applications	IHC-P ICC/IF ICC/IF WB FC
Species Reactivities	Mouse, Rat, Human
Antigen	Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human HSPA4 aa 700-800
Description	Rabbit Monoclonal
Gene	HSPA4
Conjugate	Unconjugated
Supplier Page	Shop

Product images

All lanes : Anti-HSPA4 antibody [EPR14166] (ab185962) at 1/10000 dilutionLane 1 : Human cerebellum lysateLane 2 : HepG2 cell lysateLane 3 : K562 cell lysateLane 4 : HeLa cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Immunofluorescence analysis of -20°C acetone-fixed HeLa cells labeling HSPA4 with ab185962 at 1/250 dilution. Goat anti-rabbit IgG (Alexa Fluor® 555) at 1/200 dilution was used as the secondary antibody (red). Slide is counter stained with Dapi (blue).

All lanes : Anti-HSPA4 antibody [EPR14166] (ab185962) at 1/1000 dilutionLane 1 : Mouse heart lysateLane 2 : Rat brain lysateLane 3 : Rat heart lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling HSPA4 with ab185962 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

Immunofluorescence analysis of 4% paraformaldehyde-fixed HepG2 cells labeling HSPA4 with ab185962 at 1/250 dilution. Goat anti-rabbit IgG (Alexa Fluor® 555) at 1/200 dilution was used as the secondary antibody (red). Slide is counter stained with Dapi (blue).

Flow Cytometric analysis of 2% paraformaldehyde fixed HeLa cells labeling HSPA4 with ab185962 at 1/200 dilution (red) compared with a rabbit monoclonal IgG isotype control (green). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.