Anti-Hsp90 beta antibody [E296] (ab32568) at 1/100000 dilution (purified) + SH-S5SY cell lysate at 20 µgSecondaryHRP goat anti-rabbit (H+L) at 1/1000 dilution

All lanes : Anti-Hsp90 beta antibody [E296] (ab32568) at 1/100000 dilution (purified)Lane 1 : HeLa cell lysateLane 2 : Jurkat cell lysateLane 3 : Raji cell lysateLane 4 : A431 cell lysateLysates/proteins at 20 µg per lane.SecondaryHRP goat anti-rabbit (H+L) at 1/1000 dilution

Immunohistochemical staining of paraffin embedded human stomach with purified ab32568 at a working dilution of 1 in 150. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

Immunofluorescence staining of HepG2 cells with purified ab32568 at a working dilution of 1 in 100, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 555 goat anti rabbit (ab150082), used at a dilution of 1 in 400. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative control is shown in bottom right hand panel - for the negative control, purified ab32568 was used at a dilution of 1/200 followed by an Alexa Fluor® 488 goat anti-mouse antibody at a dilution of 1/500.

Anti-Hsp90 beta antibody [E296] (ab32568) at 1/500 dilution (unpurified) + Hela cell lysate

Immunohistochemical analysis of paraffin-embedded urinary bladder carcinoma using unpurified ab32568 at 1/50 dilution.Immunohistochemical analysis of paraffin-embedded urinary bladder carcinoma using ab32568 at 1/50 dilution.