Anti-HOMER3 antibody (ab175852) at 1/1000 dilution + MDA-MB231 cell line lysate at 35 µg
Anti-HOMER3 antibody (ab175852) at 1/1000 dilution + NIH3T3 cell line lysate at 35 µg
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human brain tissue labeling HOMER3 with ab175852 at 1/10 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of SK-Br-3 cells labeling HOMER3 with ab175852 at 1/10 dilution (bottom histogram) compared to negative control cells (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
All lanes : Anti-HOMER3 antibody (ab175852) at 1/1000 dilutionLane 1 : Non-transfected 293 cell lysateLane 2 : 293 cell lysate transiently transfected with the HOMER3 geneLysates/proteins at 2 µg per lane.