
Anti-HMBS antibody (ab55166) at 5 µg/ml + 0.2 µg/lane of full length recombinant HMBS protein with a tag. SecondaryGoat anti mouse IgG (H&L)-HRP conjugate at 1/5000 dilution

All lanes : Anti-HMBS antibody (ab55166) at 5 µg/mlLane 1 : 50 µg/lane of transfected 293T cell line lysateLane 2 : 50 µg/lane of non-transfected 293T cell line lysateSecondaryGoat anti mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

ab55166, staining HMBS by Elisa. The detection limit for recombinant tagged HMBS is approximately 1ng/ml as a capture antibody.