All lanes : Anti-HLA-DQA1 antibody - N-terminal (ab170555) at 1/100 dilutionLane 1 : NCI-H460 cell line lysate, non-transfected with the HLA-DQA1 geneLane 2 : NCI-H460 cell line lysate, transiently transfected with the HLA-DQA1 geneLysates/proteins at 2 µg per lane.
Anti-HLA-DQA1 antibody - N-terminal (ab170555) at 1/100 dilution + NCI-H460 cell line lysate at 35 µg
Flow cytometric analysis of NCI-H460 cells labeling HLA-DQA1 with ab170555 at 1/10 dilution (right histogram), compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.