Anti-HLA Class I antibody [W6/32.]
| Name | Anti-HLA Class I antibody [W6/32.] |
|---|---|
| Supplier | Abcam |
| Catalog | ab7855 |
| Prices | $385.00 |
| Sizes | 500 µl |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2a |
| Clone | W6/32. |
| Applications | IHC-F FRET-assay FC ICC/IF ICC/IF |
| Species Reactivities | Human |
| Antigen | The cell membranes of human tonsil lymphocytes |
| Description | Mouse Monoclonal |
| Gene | HLA-A |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab7855 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7855, 1/200 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
![Overlay histogram showing Jurkat cells stained with ab7855 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab7855, 0.5µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Tween used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/2543_HLA-Class-I-Primary-antibodies-ab7855-2.jpg)
Overlay histogram showing Jurkat cells stained with ab7855 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab7855, 0.5µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Tween used under the same conditions.
Product References
PI3KC2alpha, a class II PI3K, is required for dynamin-independent internalization - PI3KC2alpha, a class II PI3K, is required for dynamin-independent internalization
Krag C, Malmberg EK, Salcini AE. J Cell Sci. 2010 Dec 15;123(Pt 24):4240-50.
The A subunit of type IIb enterotoxin (LT-IIb) suppresses the proinflammatory - The A subunit of type IIb enterotoxin (LT-IIb) suppresses the proinflammatory
Liang S, Wang M, Triantafilou K, Triantafilou M, Nawar HF, Russell MW, Connell TD, Hajishengallis G. J Immunol. 2007 Apr 15;178(8):4811-9.
Ganglioside GD1a is an essential coreceptor for Toll-like receptor 2 signaling in - Ganglioside GD1a is an essential coreceptor for Toll-like receptor 2 signaling in
Liang S, Wang M, Tapping RI, Stepensky V, Nawar HF, Triantafilou M, Triantafilou K, Connell TD, Hajishengallis G. J Biol Chem. 2007 Mar 9;282(10):7532-42. Epub 2007 Jan 16.
Differential interactions of fimbriae and lipopolysaccharide from Porphyromonas - Differential interactions of fimbriae and lipopolysaccharide from Porphyromonas
Hajishengallis G, Tapping RI, Harokopakis E, Nishiyama S, Ratti P, Schifferle RE, Lyle EA, Triantafilou M, Triantafilou K, Yoshimura F. Cell Microbiol. 2006 Oct;8(10):1557-70.
Complex interplay of activating and inhibitory signals received by Vgamma9Vdelta2 - Complex interplay of activating and inhibitory signals received by Vgamma9Vdelta2
Trichet V, Benezech C, Dousset C, Gesnel MC, Bonneville M, Breathnach R. J Immunol. 2006 Nov 1;177(9):6129-36.
Pathway of apoptosis induced in Jurkat T lymphoblasts by anti-HLA class I - Pathway of apoptosis induced in Jurkat T lymphoblasts by anti-HLA class I
Daniel D, Opelz G, Mulder A, Kleist C, Susal C. Hum Immunol. 2004 Mar;65(3):189-99.
MHC-class I antigen expression on micrometastases in bone marrow of patients with - MHC-class I antigen expression on micrometastases in bone marrow of patients with
Andratschke M, Pauli C, Stein M, Chaubal S, Wollenberg B. Anticancer Res. 2003 Mar-Apr;23(2B):1467-71.
Total loss of MHC class I in colorectal tumors can be explained by two molecular - Total loss of MHC class I in colorectal tumors can be explained by two molecular
Cabrera CM, Jimenez P, Cabrera T, Esparza C, Ruiz-Cabello F, Garrido F. Tissue Antigens. 2003 Mar;61(3):211-9.
Anti-HLA class I antibody binding to airway epithelial cells induces production - Anti-HLA class I antibody binding to airway epithelial cells induces production
Jaramillo A, Smith CR, Maruyama T, Zhang L, Patterson GA, Mohanakumar T. Hum Immunol. 2003 May;64(5):521-9.
Detection of soluble HLA class I antigens by ELISA and determination of its - Detection of soluble HLA class I antigens by ELISA and determination of its
Wu T, Lan JC, Zhang H, Wang CR, Mao GC. Di Yi Jun Yi Da Xue Xue Bao. 2002 Oct;22(10):931-3.