![All lanes : Anti-Histone H2A.Z antibody [4A4] (ab80150) at 1 µg/mlLane 1 : Histone H2A.Z recombinant proteinLane 2 : Histone H2A recombinant proteinLysates/proteins at 0.5 µg per lane.SecondaryGoat polyclonal to Rat IgG - H&L - Pre-Adsorbed (HRP) at 1/10000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/1221_Histone-H2AZ-Primary-antibodies-ab80150-1.jpg)
All lanes : Anti-Histone H2A.Z antibody [4A4] (ab80150) at 1 µg/mlLane 1 : Histone H2A.Z recombinant proteinLane 2 : Histone H2A recombinant proteinLysates/proteins at 0.5 µg per lane.SecondaryGoat polyclonal to Rat IgG - H&L - Pre-Adsorbed (HRP) at 1/10000 dilution
ab80150 (1/1000) staining Histone H2A.Z in paraffin-embedded Human pancreatic cancer tissue. Tissue underwent fixation in formaldehyde, peroxidase blocking, protein blocking and heat mediated antigen retrieval. After the primary antibody incubation a rabbit anti-rat link was used before the application of the secondary antibody which was goat anti rabbit/mouse conjugated to HRP. For further experimental details please refer to abreview.See Abreview
![Overlay histogram showing HeLa cells stained with ab80150 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab80150, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rat IgG (H+L) (ab98386) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rat IgG2b [RTK4530] ab18541, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/1223_Histone-H2AZ-Primary-antibodies-ab80150-9.jpg)
Overlay histogram showing HeLa cells stained with ab80150 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab80150, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rat IgG (H+L) (ab98386) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rat IgG2b [RTK4530] ab18541, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.