Anti-Histone H2A.Z (acetyl K4 + K7 + K11) antibody - ChIP Grade
| Name | Anti-Histone H2A.Z (acetyl K4 + K7 + K11) antibody - ChIP Grade |
|---|---|
| Supplier | Abcam |
| Catalog | ab18262 |
| Prices | $401.00 |
| Sizes | 100 µg |
| Host | Sheep |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ChIP ICC/IF ICC/IF WB |
| Species Reactivities | Mouse, Chicken, Human |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Chicken H2A |
| Description | Sheep Polyclonal |
| Gene | H2AFZ |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ChIP analysis of Acetyl-H2A.Z at the p21 transcription start site with or without the HDAC inhibitor TSA (50ng/ml). Chromatin was prepared from MDA-MB231 cells. Cells were fixed with formaldehyde for 10min. Samples were sonicated to generate DNA fragments between 500 and 700 bp. The ChIP was performed with 50µg of chromatin, fragments were immunoprecipitated using 2µg of antibody ab18262, and an HA antibody was used as control. The precipitated DNA was amplified by real-time PCR, with primer sets designed to amplify the promoter and the coding region of the p21 gene.
Anti-Histone H2A.Z (acetyl K4 + K7 + K11) antibody - ChIP Grade (ab18262) at 1/1000 dilution + HeLa whole cell lysate at 10 µgSecondaryHRP-conjugated donkey anti-sheep IgG polyclonal at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
ab18262 staining Histone H2A.Z (acetyl K4 + K7 + K11) in HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton X-100 and blocked with 3% BSA for 1 hour at 22°C. Samples were incubated with primary antibody (1/500 in PBS + 1% BSA) for 1 hour at 22°C. An Alexa Fluor® 488-conjugated donkey anti-sheep IgG polyclonal (1/1000) was used as the secondary antibody.See Abreview
All lanes : Anti-Histone H2A.Z (acetyl K4 + K7 + K11) antibody - ChIP Grade (ab18262)Lane 1 : Butyrate-treated HeLa histonesLane 2 : 15-day embryo chicken erythrocyte histonesLane 3 : Recombinant H2A.ZLane 4 : Butyrate-treated HeLa histonesLane 5 : 15-day embryo chicken erythrocyte histonesLane 6 : Recombinant H2A.Z
All lanes : Anti-Histone H2A.Z (acetyl K4 + K7 + K11) antibody - ChIP Grade (ab18262)Lane 1 : Butyrate-treated HeLa histonesLane 2 : Nucleosomes from 10-day chicken brain tissue. ChIP Input.Lane 3 : Nucleosomes from 10-day chicken brain tissue. ChIP Unbound.Lane 4 : Nucleosomes from 10-day chicken brain tissue. ChIP Bound.Lane 5 : Butyrate-treated HeLa histonesLane 6 : Nucleosomes from 10-day chicken brain tissue. ChIP Input.Lane 7 : Nucleosomes from 10-day chicken brain tissue. ChIP Unbound.Lane 8 : Nucleosomes from 10-day chicken brain tissue. ChIP Bound.
Product References
Histone H3 lysine 4 trimethylation regulates cotranscriptional H2A variant - Histone H3 lysine 4 trimethylation regulates cotranscriptional H2A variant
Kusch T, Mei A, Nguyen C. Proc Natl Acad Sci U S A. 2014 Apr 1;111(13):4850-5. doi:
Genome-wide binding of MBD2 reveals strong preference for highly methylated loci. - Genome-wide binding of MBD2 reveals strong preference for highly methylated loci.
Menafra R, Brinkman AB, Matarese F, Franci G, Bartels SJ, Nguyen L, Shimbo T, Wade PA, Hubner NC, Stunnenberg HG. PLoS One. 2014 Jun 13;9(6):e99603.
Histone H2A.Z subunit exchange controls consolidation of recent and remote - Histone H2A.Z subunit exchange controls consolidation of recent and remote
Zovkic IB, Paulukaitis BS, Day JJ, Etikala DM, Sweatt JD. Nature. 2014 Nov 27;515(7528):582-6.
TIP48/Reptin and H2A.Z requirement for initiating chromatin remodeling in - TIP48/Reptin and H2A.Z requirement for initiating chromatin remodeling in
Dalvai M, Fleury L, Bellucci L, Kocanova S, Bystricky K. PLoS Genet. 2013 Apr;9(4):e1003387.
H2A.Z-dependent crosstalk between enhancer and promoter regulates cyclin D1 - H2A.Z-dependent crosstalk between enhancer and promoter regulates cyclin D1
Dalvai M, Bellucci L, Fleury L, Lavigne AC, Moutahir F, Bystricky K. Oncogene. 2013 Sep 5;32(36):4243-51.
Activation of p21 by HDAC inhibitors requires acetylation of H2A.Z. - Activation of p21 by HDAC inhibitors requires acetylation of H2A.Z.
Bellucci L, Dalvai M, Kocanova S, Moutahir F, Bystricky K. PLoS One. 2013;8(1):e54102.
Regulation of transcription through acetylation of H3K122 on the lateral surface - Regulation of transcription through acetylation of H3K122 on the lateral surface
Tropberger P, Pott S, Keller C, Kamieniarz-Gdula K, Caron M, Richter F, Li G, Mittler G, Liu ET, Buhler M, Margueron R, Schneider R. Cell. 2013 Feb 14;152(4):859-72.
Integrative analysis of deep sequencing data identifies estrogen receptor early - Integrative analysis of deep sequencing data identifies estrogen receptor early
Ovaska K, Matarese F, Grote K, Charapitsa I, Cervera A, Liu C, Reid G, Seifert M, Stunnenberg HG, Hautaniemi S. PLoS Comput Biol. 2013;9(6):e1003100.
Acetylation of H2A.Z is a key epigenetic modification associated with gene - Acetylation of H2A.Z is a key epigenetic modification associated with gene
Valdes-Mora F, Song JZ, Statham AL, Strbenac D, Robinson MD, Nair SS, Patterson KI, Tremethick DJ, Stirzaker C, Clark SJ. Genome Res. 2012 Feb;22(2):307-21.
Histone crosstalk directed by H2B ubiquitination is required for chromatin - Histone crosstalk directed by H2B ubiquitination is required for chromatin
Ma MK, Heath C, Hair A, West AG. PLoS Genet. 2011 Jul;7(7):e1002175.