Anti-Histone H1.3 (phospho T17) + Histone H1.4 (phospho T17) antibody [EPR18087]

Name	Anti-Histone H1.3 (phospho T17) + Histone H1.4 (phospho T17) antibody [EPR18087]
Supplier	Abcam
Catalog	ab188294
Prices	$99.00
Sizes	10 µl
Host	Rabbit
Clonality	Monoclonal
Isotype	IgG
Clone	EPR18087
Applications	IHC-P ICC/IF ICC/IF WB
Species Reactivities	Mouse, Rat, Human
Antigen	Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Histone H1
Description	Rabbit Monoclonal
Gene	HIST1H1D
Conjugate	Unconjugated
Supplier Page	Shop

Product images

All lanes : Anti-Histone H1.3 (phospho T17) + Histone H1.4 (phospho T17) antibody [EPR18087] (ab188294) at 1/5000 dilutionLane 1 : Untreated HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysatesLane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma) treated with Colcemid acid extraction lysatesLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

All lanes : Anti-Histone H1.3 (phospho T17) + Histone H1.4 (phospho T17) antibody [EPR18087] (ab188294) at 1/5000 dilutionLane 1 : Untreated NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysatesLane 2 : NIH/3T3 (Mouse embyro fibroblast cells) treated with 1.5µg/ml Colcemid for 12 hours whole cell lysatesLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Histone H1.3 (phospho T17) + Histone H1.4 (phospho T17) with ab188294 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution.Nucleus staining on epithelial cells of Human colon is observed.Counter stained with Hematoxylin.Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling Histone H1.3 (phospho T17) + Histone H1.4 (phospho T17) with ab188294 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution.Nucleus staining on epithelial cells of mouse colon is observed.Counter stained with Hematoxylin.Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling Histone H1.3 (phospho T17) + Histone H1.4 (phospho T17) with ab188294 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution.Nucleus staining on epithelial cells of rat colon is observed.Counter stained with Hematoxylin.Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling Histone H1.3 (phospho T17) + Histone H1.4 (phospho T17) with ab188294 at 1/8000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green).Confocal image showing nuclear staining on HeLa cell line.The nuclear counter stain is DAPI (blue).Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).The negative controls are as follows:-ve control 1: ab188294 at 1/8000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.