Anti-Histone H1.2 antibody [EPR12690]

Name	Anti-Histone H1.2 antibody [EPR12690]
Supplier	Abcam
Catalog	ab181973
Prices	$384.00
Sizes	100 µl
Host	Rabbit
Clonality	Monoclonal
Isotype	IgG
Clone	EPR12690
Applications	ChIP WB IHC-P ICC/IF ICC/IF
Species Reactivities	Mouse, Rat, Human
Antigen	Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Histone H1
Description	Rabbit Monoclonal
Gene	HIST1H1C
Conjugate	Unconjugated
Supplier Page	Shop

Product images

ChIP analysis using ab181973 binding Histone H1.2 in mouse primary hepatocytes. Cells were cross-linked for 10 minutes with 1% formaldehyde. Samples were incubated with the primary antibody (1/100) for 20 hours at 4°C. Protein binding was detected using real-time PCR.Negative Control: IgG.See Abreview

All lanes : Anti-Histone H1.2 antibody [EPR12690] (ab181973) at 1/10000 dilutionLane 1 : HeLa cell lysateLane 2 : A375 cell lysateLane 3 : 293 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Anti-Histone H1.2 antibody [EPR12690] (ab181973) at 1/2000 dilution + MCF7 cell lysate at 20 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling Histone H1.2 with ab181973 at 1/100 dilution, followed by prediluted ImmunoHistoprobe (Ready to use) HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

Immunohistochemical analysis of paraffin-embedded Mouse muscle tissue labeling Histone H1.2 with ab181973 at 1/100 dilution, followed by prediluted ImmunoHistoprobe (Ready to use) HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

Immunofluorescent analysis of 4% paraformaldehyde-fixed MCF7 cells labeling Histone H1.2 with ab181973 at 1/250 dilution, followed by Goat anti rabbit IgG (Dylight 488) at 1/200 dilution (green), Dapi staining (blue).