Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human liver cancer tissue sections labeling HIF-1-alpha with ab113642 at 1/200 with DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human lung cancer tissue sections labeling HIF-1-alpha with ab113642 at 1/200 with DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human stomach cancer tissue sections labeling HIF-1-alpha using ab113642 at 1/200 with DAB staining.

Immunohistochemistry (Formalin-PFA-fixed paraffin-embedded sections) analysis Human brain tumor tissue sections labeling HIF-1-alpha with ab113642 at 1/200 with DAB staining.

All lanes : Anti-HIF-1-alpha antibody [1A3] (ab113642) at 1/500 dilutionLane 1 : Cos7 cell lysateLane 2 : HeLa cell lysateLane 3 : Jurkat cell lysateLane 4 : Raji cell lysateLane 5 : NIH 3T3 cell lysate

Overlay histogram showing HeLa cells stained with ab113642 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab113642, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Elisa using ab113642