Immunohistochemistical detection of Anti-Heme Oxygenase 1 antibody (ab85309; 1/5000 for 2h @ 21°C) on human lung formalin/PFA-fixed paraffin-embedded sections. Antigen retrieval step:Heat mediated - Buffer/Enzyme Used: Citric acid.Blocking step: 1% BSA for 10 mins at 21°C. Secondary antibody biotin conjugated anti Rabbit IgG (1/200). NB: Positivity appears to be confined to macrophages- the primary antibody was omitted on the sequential section and was negative for macrophages ( to rule out lipofuscin).See Abreview
All lanes : Anti-Heme Oxygenase 1 antibody (ab85309) at 1 µg/mlLane 1 : Spleen (Rat) Tissue Lysate Lane 2 : Heart (Rat) Tissue Lysate Lane 3 : Lung (Rat) Tissue Lysate Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
All lanes : Anti-Heme Oxygenase 1 antibody (ab85309) at 1 µg/mlLane 1 : Spleen (Human) Tissue Lysate - adult normal tissue (ab29699)Lane 2 : Lung (Human) Whole Cell Lysate - adult normal tissue (ab30281)Lane 3 : Heart (Mouse) Tissue Lysate Lane 4 : Spleen (Mouse) Tissue LysateLane 5 : Lung (Mouse) Whole Cell Lysate - normal tissue (ab29297)Lysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
Immunohistochemistical detection of Anti-Heme Oxygenase 1 antibody (ab85309; 1/5000 for 2h @ 21°C)on rat lung formalin/PFA-fixed paraffin-embedded sections. Antigen retrieval step:Heat mediated - Buffer/Enzyme Used: Citric acid.Blocking step: 1% BSA for 10 mins. Secondary antibody biotin conjugated anti Rabbit IgG (1/200). NB: As well as in macrophage cytoplasm, there is also clear positivity in the lining epitheium of the respiratory conducting tubes. I do not know if this is specific/non-specific.