Anti-HDAC4 antibody - ChIP Grade
| Name | Anti-HDAC4 antibody - ChIP Grade |
|---|---|
| Supplier | Abcam |
| Catalog | ab1437 |
| Prices | $394.00 |
| Sizes | 50 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-F ICC/IF ICC/IF WB ChIP |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | This information is considered to be commercially sensitive(Peptide available as ab53331 |
| Blocking Peptide | HDAC4 peptide |
| Description | Rabbit Polyclonal |
| Gene | HDAC4 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab1437 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab1437, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
All lanes : Anti-HDAC4 antibody - ChIP Grade (ab1437) at 1/500 dilutionLane 1 : HDAC4 KO whole mouse brain (P3)Lane 2 : WT whole mouse brain (P3)Lysates/proteins at 30 µg per lane.SecondaryAnti-Rabbit IgG, HRP-conjugated F(ab')2 Fragment at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
Product References
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Modulation of chromatin position and gene expression by HDAC4 interaction with - Modulation of chromatin position and gene expression by HDAC4 interaction with
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miR-10a contributes to retinoid acid-induced smooth muscle cell differentiation. - miR-10a contributes to retinoid acid-induced smooth muscle cell differentiation.
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Differential regulation of HIC1 target genes by CtBP and NuRD, via an - Differential regulation of HIC1 target genes by CtBP and NuRD, via an
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Recruitment of CREB1 and histone deacetylase 2 (HDAC2) to the mouse Ltbp-1 - Recruitment of CREB1 and histone deacetylase 2 (HDAC2) to the mouse Ltbp-1
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