Immunohistochemical analysis of formalin fixed, paraffin embedded Human ovarian carcinoma tissue labeling HCE using ab176728 at a 1/200 dilution (1µg/ml), using peroxidase substrate for detection.
Western blot of immunopricipitate of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), labeling HCE using ab176728 for WB at 0.4 µg/ml and used for IP at 3 µg/mg lysate.
All lanes : Anti-HCE antibody (ab176728) at 0.04 µg/mlLane 1 : HeLa whole cell lysate at 50 µgLane 2 : HeLa whole cell lysate at 15 µgLane 3 : HeLa whole cell lysate at 5 µgLane 4 : 293T whole cell lysate at 50 µgLane 5 : NIH3T3 whole cell lysate at 50 µgdeveloped using the ECL technique