
All lanes : Anti-HB9/HLXB9 antibody (ab94360) at 1 µg/mlLane 1 : PANC-1 (Human pancreatic carcinoma, epithelial-like cell line) Nuclear Lysate (ab14882)Lane 2 : U937 (Human leukemic monocyte lymphoma cell line) Whole Cell LysateLane 3 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell LysateLane 4 : MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.