
All lanes : Anti-HADHA antibody (ab137831) at 1/1000 dilutionLane 1 : 293T whole cell lysateLane 2 : A431 whole cell lysateLysates/proteins at 30 µg per lane.

Anti-HADHA antibody (ab137831) at 1/1000 dilution + Mouse liver whole cell lysate at 50 µg

Anti-HADHA antibody (ab137831) at 1/500 dilution + PC12 whole cell lysate at 30 µg

Immunohistochemical analysis of paraffin embedded colon carcinoma tissue, labelling HADHA using ab137831 at 1/250 dilution.

Immunofluorescence analysis of methanol fixed (100% methanol at -20 °C for 5 minutes) HepG2 cells, labelling HADHA using ab137831 at 1/500 dilution. Right panel (blue) shows Hoechst 33343 staining.