![All lanes : Anti-Granulin antibody [EPR3781] (ab108608) at 1/1000 dilutionLane 1 : A431 cell lysateLane 2 : 293T cell lysateLane 3 : MCF7 cell lysateLane 4 : HeLa cell lysateLane 5 : Human fetal lung tissue lysateLane 6 : Human fetal kidney tissue lysateLysates/proteins at 10 µg per lane.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/27363_Granulin-Primary-antibodies-ab108608-1.jpg)
All lanes : Anti-Granulin antibody [EPR3781] (ab108608) at 1/1000 dilutionLane 1 : A431 cell lysateLane 2 : 293T cell lysateLane 3 : MCF7 cell lysateLane 4 : HeLa cell lysateLane 5 : Human fetal lung tissue lysateLane 6 : Human fetal kidney tissue lysateLysates/proteins at 10 µg per lane.
Immunohistochemical analysis of paraffin-embedded Human kidney tissue using ab108608 at 1/100.
ICC/IF image of ab108608 stained Malme-3M cells. The cells were 4% paraformaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab108608, 1/200 dilution) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.