All lanes : Anti-Glutathione S Transferase alpha antibody (ab96043) at 1 µg/mlLane 1 : Liver (Human) Tissue Lysate - adult normal tissue (ab29889)Lane 2 : Liver (Rat) Tissue LysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
All lanes : Anti-Glutathione S Transferase alpha antibody (ab96043) at 1 µg/mlLane 1 : Human Glutathione S Transferase alpha full length protein (ab95381) at 0.1 µgLane 2 : Human Glutathione S Transferase alpha full length protein (ab95381) at 0.01 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.Exposure time : 8 minutes