
Anti-GLO1 antibody (ab135592) at 1/50 dilution + HL60 cell line lysate at 35 µg

Immunohistochemical analysis of formalin fixed, paraffin embedded Human brain tissue labelling GLO1 with ab135592 at a 1/50 dilution, followed by peroxidase conjugation of the secondary antibody and DAB detection.

Flow cytometric analysis of WiDr cells (bottom histogram)labelling GLO1 with ab135592 at 1/10 dilution, compared to a negative control cell (top histogram). FITC-conjugated Goat-anti-Rabbit secondary antibodies were used for the analysis.

All lanes : Anti-GLO1 antibody (ab135592) at 1/50 dilutionLane 1 : Non transfected 293 cell lysates Lane 2 : 293 cell lysates transiently transfected with GLO1 Lysates/proteins at 2 µg per lane.

Immunofluorescence analysis of WiDr cells labelling GLO1 with ab135592 at a 1/10 dilution, followed by Alexa Fluor 488-conjugated Goat anti-Rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).