![Standard Curve for GFAP; dilution range 1 pg/ml to 1 ug/ml using Capture Antibody Mouse monoclonal [2A5] to GFAP - Astrocyte Marker (ab4648) at 0.2 ug/ml and Detector Antibody Rabbit polyclonal to GFAP - Astrocyte Marker (ab48050) at 0.5 ug/ml.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/24109_GFAP-Primary-antibodies-ab48050-8.jpg)
Standard Curve for GFAP; dilution range 1 pg/ml to 1 ug/ml using Capture Antibody Mouse monoclonal [2A5] to GFAP - Astrocyte Marker (ab4648) at 0.2 ug/ml and Detector Antibody Rabbit polyclonal to GFAP - Astrocyte Marker (ab48050) at 0.5 ug/ml.

Immunoflourescent staining of mouse brain using anti-GFAP (ab 48050). Mouse tissues were fixed (animals perfused) with 4% PFA and later postfixed overnight in the same fixative. They were cryoprotected in 30% sucrose and cut with microtome (25 µm thickness). Immunofuorescent staining performed on mouse brain sections blocked with 10% serum over 1 hour using the anti-GFAP primary antibody (ab 48050) at 1/200 for 24 hours at 4°C. Incubation with anti-rabbit Alexa 488 at 1/1000 followed. The antibody stains astrocytes and nucleus stained with Hoechst at high power magnification.See Abreview

Immunohistochemical staining of rat cerebellum using anti-GFAP (ab 48050). Tissues were fixed with formaldehyde and antigen retrieved using heat mediated citric acid buffer. Immunohistochemical analysis was performed on rat cerebellum sections (blocked with 1% BSA for 10 minutes) using the anti-GFAP primary antibody (ab48050) at 1/2000 for 2 hours at 21°C. This was followed by incubation with anti-rabbit biotin conjugated secondary anbibody at 1/200. Astrocytes appear to be specifically immunolabelled.See Abreview

All lanes : Anti-GFAP antibody - Astrocyte Marker (ab48050) at 1 µg/mlLane 1 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)Lane 2 : Brain (Rat) Rat Tissue LysateLysates/proteins at 10 µg per lane.SecondaryIRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilutionPerformed under reducing conditions.

Fluorescent immunocytochemistry staining of cultured rat astrocytes using anti-GFAP (ab 48050). Cultured rat astrocytes were fixed with 4% PFA and permeabilized with 0.1% Triton X in 0.1% PBS. Fluorescent immunocytochemistry staining performed on cultured rat astrocytes blocked with 10% serum over 1 hour at 24ºC using anti-GFAP primary antibody (ab 48050) at 1/200 for 4 hours at 24°C. Incubation with donkey anti-rabbit Alexa 488 secondary at 1/1000 followed. Cells co-stained with BMP6 in red.See Abreview

Anti-GFAP antibody - Astrocyte Marker (ab48050) at 1 µg/ml + Human GFAP full length protein (ab114149) at 0.1 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.Exposure time : 10 seconds