ICC/IF image of ab101267 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab101267, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

All lanes : Anti-GC1q R antibody (ab101267) at 0.1 µg/mlLane 1 : HeLa whole cell lysate at 50 µg/mlLane 2 : HeLa whole cell lysate at 15 µg/mlLane 3 : HeLa whole cell lysate at 5 µg/mlLane 4 : 293T whole cell lysate at 50 µg/mlLane 5 : NIH3T3 whole cell lysate at 50 µg/mldeveloped using the ECL technique

Detection of Human GC1q R in 1mg HeLa whole cell lysate (20% of IP loaded) using ab101267 at 3µg/mg of lysate. Further Western Blot detection was performed using ab101267 at 0.4µg/ml. Lane 1: ab101267 IP. Lane 2: control IgG IP.