Anti-GATA1 antibody
| Name | Anti-GATA1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab28839 |
| Prices | $400.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB ELISA IHC-P IP ICC/IF ICC/IF |
| Species Reactivities | Mouse, Human |
| Antigen | Synthetic non-phosphopeptide derived from human GATA1 around the phosphorylation site of Serine 142 |
| Description | Rabbit Polyclonal |
| Gene | GATA1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Immunohistochemical analysis of paraffin-embedded breast carcinoma. Left: Using GATA-1(Ab-1) Antibody (ab28839); Right: The same antibody preincubated with synthesized peptide.
All lanes : Anti-GATA1 antibody (ab28839) (1/500-1/1000 dilution)Lane 1 : extracts from K562 cells (5-30ug).Lane 2 : extracts from K562 cells (5-30ug).
ICC/IF image of ab28839 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28839, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
A multiply redundant genetic switch 'locks in' the transcriptional signature of - A multiply redundant genetic switch 'locks in' the transcriptional signature of
Fu W, Ergun A, Lu T, Hill JA, Haxhinasto S, Fassett MS, Gazit R, Adoro S, Glimcher L, Chan S, Kastner P, Rossi D, Collins JJ, Mathis D, Benoist C. Nat Immunol. 2012 Oct;13(10):972-80.
The transcription factor GATA-1 is overexpressed in breast carcinomas and - The transcription factor GATA-1 is overexpressed in breast carcinomas and
Boidot R, Vegran F, Jacob D, Chevrier S, Cadouot M, Feron O, Solary E, Lizard-Nacol S. Oncogene. 2010 Apr 29;29(17):2577-84.
New GATA1 mutation in codon 2 leads to the earliest known premature stop codon in - New GATA1 mutation in codon 2 leads to the earliest known premature stop codon in
Hoeller S, Bihl MP, Tzankov A, Kuehne T, Potthoff C, Bruder E. Blood. 2009 Oct 22;114(17):3717-8.
Transcriptional coactivator p300 regulates glucose-induced gene expression in - Transcriptional coactivator p300 regulates glucose-induced gene expression in
Chen S, Feng B, George B, Chakrabarti R, Chen M, Chakrabarti S. Am J Physiol Endocrinol Metab. 2010 Jan;298(1):E127-37. doi: