ICC/IF image of ab42905 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab42905, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

ab42905 staining GARS in murine sciatic nerve tissue by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue was fixed with paraformaldehyde and blocked with 5% goat serum in PBS with 0.5% Triton X-100 for 1 hour at room temperature. Samples were incubated with primary antibody (1/500 in diluent) overnight at 4°C. An AlexaFluor®555-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody.