Anti-Galectin 3 antibody [A3A12]

Name	Anti-Galectin 3 antibody [A3A12]
Supplier	Abcam
Catalog	ab2785
Prices	$403.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Isotype	IgG1
Clone	A3A12
Applications	ELISA IHC-F WB ICC/IF ICC/IF B/N IHC-P ICC/IF
Species Reactivities	Mouse, Rat, Rabbit, Human
Antigen	Recombinant full length protein corresponding to Human Galectin 3
Description	Mouse Monoclonal
Gene	LGALS3
Conjugate	Unconjugated
Supplier Page	Shop

Product images

ab2785 labelling Galectin 3 (green) in the cytoplasm and nucleus of HeLa cells by Immunocytochemistry/Immunofluorescence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:100 in 3% BSA-PBS) overnight at 4 ºC. A DyLight-conjugated anti-mouse was used as the secondary antibody. Red (phalloidin) - F-actin, Blue - nuclei. Images were taken at a magnification of 60x.

ab2785 labelling Galectin 3 (green) in the cytoplasm and nucleus of NIH-3T3 cells by Immunocytochemistry/Immunofluorescence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:100 in 3% BSA-PBS) overnight at 4 ºC. A DyLight-conjugated anti-mouse was used as the secondary antibody. Red (phalloidin) - F-actin, Blue - nuclei. Images were taken at a magnification of 60x.

ab2785 labelling Galectin 3 (green) in the cytoplasm and nucleus of MCF-7 cells by Immunocytochemistry/Immunofluorescence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:100 in 3% BSA-PBS) overnight at 4 ºC. A DyLight-conjugated anti-mouse was used as the secondary antibody. Red (phalloidin) - F-actin, Blue - nuclei. Images were taken at a magnification of 60x.

ab2785 labelling Galectin 3 in the nucleus and cytoplasm of Human ovarian carcinoma tissue (right) compared with a negative control (left) by Immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tissue sections were incubated with the primary antibody (1:100 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-mouse IgG was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

ab2785 labelling Galectin 3 in the nucleus and cytoplasm of Human colon tissue (right) compared with a negative control (left) by Immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tissue sections were incubated with the primary antibody (1:100 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-mouse IgG was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

ab2785 labelling Galectin 3 in the nucleus and cytoplasm of Mouse colon tissue (right) compared with a negative control (left) by Immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tissue sections were incubated with the primary antibody (1:20 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-mouse IgG was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

All lanes : Anti-Galectin 3 antibody [A3A12] (ab2785) at 1/1000 dilutionLane 1 : MCF-7 cell lysateLane 2 : HeLa cell lysateLane 3 : NIH-3T3 cell lysateLysates/proteins at 25 µg/ml per lane.

ICC/IF image of ab2785 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2785, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

ab2785 staining Galectin 3 in rat spleen tissue by Immunohistochemistry (Frozen sections).Tissue was fixed in paraformaldehyde, blocked with 20% serum for 20 minutes at 24°C, then incubated with ab2785 at a 1/200 dilution for 16 hours at 4°C. The secondary used was an Alexa-Fluor 488 conjugated donkey anti-mouse polyclonal used at a 1/1000 dilution. Counterstained with Hoechst 33258 (blue).See Abreview

Product References

Epigenetic regulation of galectin-3 expression by beta1 integrins promotes cell - Epigenetic regulation of galectin-3 expression by beta1 integrins promotes cell

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Comprehensive analysis of cellular galectin-3 reveals no consistent oncogenic - Comprehensive analysis of cellular galectin-3 reveals no consistent oncogenic

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Aberrant epithelial morphology and persistent epidermal growth factor receptor - Aberrant epithelial morphology and persistent epidermal growth factor receptor

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Expression of the transcription factor NFAT2 in human neutrophils: IgE-dependent, - Expression of the transcription factor NFAT2 in human neutrophils: IgE-dependent,

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RNA interference-mediated choline kinase suppression in breast cancer cells - RNA interference-mediated choline kinase suppression in breast cancer cells

Glunde K, Raman V, Mori N, Bhujwalla ZM. Cancer Res. 2005 Dec 1;65(23):11034-43.

Modulation of functional properties of galectin-3 by monoclonal antibodies - Modulation of functional properties of galectin-3 by monoclonal antibodies

Liu FT, Hsu DK, Zuberi RI, Hill PN, Shenhav A, Kuwabara I, Chen SS. Biochemistry. 1996 May 14;35(19):6073-9.